Review



pmir report luc klf4 fl vector  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc pmir report luc klf4 fl vector
    Pmir Report Luc Klf4 Fl Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmir+report+luc+klf4+fl+vector/pMIR-Report-Luc-KLF4-FL+(Plasmid+%2334597)/pm37569807-270-1-8
    Average 93 stars, based on 8 article reviews
    pmir report luc klf4 fl vector - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Inhibition of miR-25 Ameliorates Cardiac Dysfunction and Fibrosis by Restoring KLF4 Expression
    Article Snippet: .. The pMIR-Report-Luc-KLF4-FL vector (Plasmid #34597) was purchased from Addgene (MA, USA). ..

    Article Title: Dual Epigenetic Regulation of ERα36 Expression in Breast Cancer Cells
    Article Snippet: .. Reporter plasmids containing the miR-136-5p target sequence from ERα36 3’UTR downstream the luciferase open reading frame were constructed as follows: pMIR-Report-Luc-KLF4-FL vector was purchased from the nonprofit Addgene repository (Addgene plasmid #34597) as a gift from Michael Ruppert [ ]. ..

    Article Title: Inhibition of miR-25 Ameliorates Cardiac Dysfunction and Fibrosis by Restoring Krüppel-like Factor 4 Expression
    Article Snippet: .. The pMIR-Report-Luc-KLF4-FL vector (Plasmid #34597) was purchased from Addgene (Watertown, MA, USA). ..

    Article Title: Inhibition of miR-25 Ameliorates Cardiac Dysfunction and Fibrosis by Restoring Krüppel-like Factor 4 Expression.
    Article Snippet: .. The pMIR-Report-Luc-KLF4-FL vector (Plasmid #34597) was purchased from Addgene (Watertown, MA, USA). ..

    Sequencing:

    Article Title: Dual Epigenetic Regulation of ERα36 Expression in Breast Cancer Cells
    Article Snippet: .. Reporter plasmids containing the miR-136-5p target sequence from ERα36 3’UTR downstream the luciferase open reading frame were constructed as follows: pMIR-Report-Luc-KLF4-FL vector was purchased from the nonprofit Addgene repository (Addgene plasmid #34597) as a gift from Michael Ruppert [ ]. ..

    Luciferase:

    Article Title: Dual Epigenetic Regulation of ERα36 Expression in Breast Cancer Cells
    Article Snippet: .. Reporter plasmids containing the miR-136-5p target sequence from ERα36 3’UTR downstream the luciferase open reading frame were constructed as follows: pMIR-Report-Luc-KLF4-FL vector was purchased from the nonprofit Addgene repository (Addgene plasmid #34597) as a gift from Michael Ruppert [ ]. ..

    Construct:

    Article Title: Dual Epigenetic Regulation of ERα36 Expression in Breast Cancer Cells
    Article Snippet: .. Reporter plasmids containing the miR-136-5p target sequence from ERα36 3’UTR downstream the luciferase open reading frame were constructed as follows: pMIR-Report-Luc-KLF4-FL vector was purchased from the nonprofit Addgene repository (Addgene plasmid #34597) as a gift from Michael Ruppert [ ]. ..



    Similar Products

    pmir report luc klf4 fl vector  (Addgene inc)
    93
    Addgene inc pmir report luc klf4 fl vector
    Pmir Report Luc Klf4 Fl Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmir+report+luc+klf4+fl+vector/pMIR-Report-Luc-KLF4-FL+(Plasmid+%2334597)/pm37569807-270-1-8
    Average 93 stars, based on 1 article reviews
    pmir report luc klf4 fl vector - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    pmir reporter vector  (Addgene inc)
    93
    Addgene inc pmir reporter vector
    TWIST1 is <t>a</t> <t>miR-186-5p</t> target. Inset: The putative binding site of miR-186-5p within the Twist1 3’-UTR is shown, with the paired sequences of the wt and mutant (mut) constructs generated. (A) The <t>pMIR-Twist1-WT</t> and pMIR-Twist1-MT vectors were transfected into the three MPM cell lines and in HEK293 cells along with miR-186-5p or ctrl mimics and luciferase activity was assessed 48 h later. The percentage of luciferase activity in the cells transfected with miR-186-5p mimics over the cells transfected with the ctrl-mimics is reported. Means ± SD of three replicates are shown. * P < 0.05, ** P < 0.01. (B) Twist1 protein levels in NCI-H2373 cells treated with ctrl or miR-186-5p mimics or butein and ctrl (DMSO 0.01 %) as assessed by Western blotting. Anti-tubulin staining was used as a loading control. Asterisk (*) indicates the TWIST1-specific protein band. (C) Histograms reporting the relative (normalized) protein band intensity of TWIST1 from all three MPM cell lines treated as indicated in (B). The average values of two independent experiments are reported. ** P < 0.01 ( vs . ctrl).
    Pmir Reporter Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmir+report+luc+klf4+fl+vector/pMIR-Report-Luc-KLF4-FL+(Plasmid+%2334597)/pmc09511809-45-16-18
    Average 93 stars, based on 1 article reviews
    pmir reporter vector - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    TWIST1 is a miR-186-5p target. Inset: The putative binding site of miR-186-5p within the Twist1 3’-UTR is shown, with the paired sequences of the wt and mutant (mut) constructs generated. (A) The pMIR-Twist1-WT and pMIR-Twist1-MT vectors were transfected into the three MPM cell lines and in HEK293 cells along with miR-186-5p or ctrl mimics and luciferase activity was assessed 48 h later. The percentage of luciferase activity in the cells transfected with miR-186-5p mimics over the cells transfected with the ctrl-mimics is reported. Means ± SD of three replicates are shown. * P < 0.05, ** P < 0.01. (B) Twist1 protein levels in NCI-H2373 cells treated with ctrl or miR-186-5p mimics or butein and ctrl (DMSO 0.01 %) as assessed by Western blotting. Anti-tubulin staining was used as a loading control. Asterisk (*) indicates the TWIST1-specific protein band. (C) Histograms reporting the relative (normalized) protein band intensity of TWIST1 from all three MPM cell lines treated as indicated in (B). The average values of two independent experiments are reported. ** P < 0.01 ( vs . ctrl).

    Journal: Cancer Drug Resistance

    Article Title: Butein-instigated miR-186-5p-dependent modulation of TWIST1 affects resistance to cisplatin and bioenergetics of Malignant Pleural Mesothelioma cells

    doi: 10.20517/cdr.2022.56

    Figure Lengend Snippet: TWIST1 is a miR-186-5p target. Inset: The putative binding site of miR-186-5p within the Twist1 3’-UTR is shown, with the paired sequences of the wt and mutant (mut) constructs generated. (A) The pMIR-Twist1-WT and pMIR-Twist1-MT vectors were transfected into the three MPM cell lines and in HEK293 cells along with miR-186-5p or ctrl mimics and luciferase activity was assessed 48 h later. The percentage of luciferase activity in the cells transfected with miR-186-5p mimics over the cells transfected with the ctrl-mimics is reported. Means ± SD of three replicates are shown. * P < 0.05, ** P < 0.01. (B) Twist1 protein levels in NCI-H2373 cells treated with ctrl or miR-186-5p mimics or butein and ctrl (DMSO 0.01 %) as assessed by Western blotting. Anti-tubulin staining was used as a loading control. Asterisk (*) indicates the TWIST1-specific protein band. (C) Histograms reporting the relative (normalized) protein band intensity of TWIST1 from all three MPM cell lines treated as indicated in (B). The average values of two independent experiments are reported. ** P < 0.01 ( vs . ctrl).

    Article Snippet: The TWIST1 3’ UTR fragment sequence containing the binding site with miR-186-5p was cloned into the pMIR-reporter vector (Addgene, Cambridge, MA).

    Techniques: Binding Assay, Mutagenesis, Construct, Generated, Transfection, Luciferase, Activity Assay, Western Blot, Staining